Grant Number:	5R21CA193067-03 Interpret this number
Primary Investigator:	Hall, Adam
Organization:	Wake Forest University Health Sciences
Project Title:	Molecular Detection of DNA Hydroxymethylation for Cancer Screening
Fiscal Year:	2017

 DESCRIPTION (provided by applicant): This project will develop a strategy to detect levels of hydroxymethylation (hmC) in genomic DNA. We will first combine a method to selectively attach a biotin to hmC with our demonstrated technique of detecting single biotin modifications in DNA to demonstrate the efficacy of our approach and optimize experimental conditions. We will then apply the technique to clinical tissue samples. First, we will measure the global hmC content of normal breast tissue and of breast carcinoma and compare our findings with results from conventional approaches. Finally, we will utilize the capability of our approach to isolate modified DNA selectively and apply the system to identifying alterations in the hmC content of a fragment of the LZTS1 gene, an important tumor suppressor.




Solid-state nanopore analysis of human genomic DNA shows unaltered global 5-hydroxymethylcytosine content associated with early-stage breast cancer.
Authors: Zahid O.K. , Rivas F. , Wang F. , Sethi K. , Reiss K. , Bearden S. , Hall A.R. .
Source: Nanomedicine : nanotechnology, biology, and medicine, 2021 Jul; 35, p. 102407.
EPub date: 2021-04-24.
PMID: 33905828
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Modular affinity-labeling of the cytosine demethylation base elements in DNA.
Authors: Wang F. , Zahid O.K. , Ghanty U. , Kohli R.M. , Hall A.R. .
Source: Scientific reports, 2020-11-20; 10(1), p. 20253.
EPub date: 2020-11-20.
PMID: 33219273
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Simple and Efficient Room-Temperature Release of Biotinylated Nucleic Acids from Streptavidin and Its Application to Selective Molecular Detection.
Authors: Bearden S. , Wang F. , Hall A.R. .
Source: Analytical chemistry, 2019-07-02; 91(13), p. 7996-8001.
EPub date: 2019-06-12.
PMID: 31144812
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Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process.
Authors: Wang F. , Zahid O.K. , Swain B.E. , Parsonage D. , Hollis T. , Harvey S. , Perrino F.W. , Kohli R.M. , Taylor E.W. , Hall A.R. .
Source: Nano letters, 2017-11-08; 17(11), p. 7110-7116.
EPub date: 2017-10-05.
PMID: 28967259
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Solid-state nanopore localization by controlled breakdown of selectively thinned membranes.
Authors: Carlsen A.T. , Briggs K. , Hall A.R. , Tabard-Cossa V. .
Source: Nanotechnology, 2017-02-24; 28(8), p. 085304-85304.
EPub date: 2017-01-03.
PMID: 28045003
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Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores.
Authors: Zahid O.K. , Zhao B.S. , He C. , Hall A.R. .
Source: Scientific reports, 2016-07-07; 6, p. 29565.
EPub date: 2016-07-07.
PMID: 27383905
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Sequence-Specific Recognition of MicroRNAs and Other Short Nucleic Acids with Solid-State Nanopores.
Authors: Zahid O.K. , Wang F. , Ruzicka J.A. , Taylor E.W. , Hall A.R. .
Source: Nano letters, 2016-03-09; 16(3), p. 2033-9.
EPub date: 2016-02-02.
PMID: 26824296
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