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Grant Details

Grant Number: 5R21CA193067-03 Interpret this number
Primary Investigator: Hall, Adam
Organization: Wake Forest University Health Sciences
Project Title: Molecular Detection of DNA Hydroxymethylation for Cancer Screening
Fiscal Year: 2017
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Abstract

? DESCRIPTION (provided by applicant): This project will develop a strategy to detect levels of hydroxymethylation (hmC) in genomic DNA. We will first combine a method to selectively attach a biotin to hmC with our demonstrated technique of detecting single biotin modifications in DNA to demonstrate the efficacy of our approach and optimize experimental conditions. We will then apply the technique to clinical tissue samples. First, we will measure the global hmC content of normal breast tissue and of breast carcinoma and compare our findings with results from conventional approaches. Finally, we will utilize the capability of our approach to isolate modified DNA selectively and apply the system to identifying alterations in the hmC content of a fragment of the LZTS1 gene, an important tumor suppressor.

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Publications

Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process.
Authors: Wang F. , Zahid O.K. , Swain B.E. , Parsonage D. , Hollis T. , Harvey S. , Perrino F.W. , Kohli R.M. , Taylor E.W. , Hall A.R. .
Source: Nano Letters, 2017-11-08 00:00:00.0; 17(11), p. 7110-7116.
EPub date: 2017-10-05 00:00:00.0.
PMID: 28967259
Related Citations

Solid-state Nanopore Localization By Controlled Breakdown Of Selectively Thinned Membranes
Authors: Carlsen A.T. , Briggs K. , Hall A.R. , Tabard-Cossa V. .
Source: Nanotechnology, 2017-02-24 00:00:00.0; 28(8), p. 085304.
PMID: 28045003
Related Citations

Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores.
Authors: Zahid O.K. , Zhao B.S. , He C. , Hall A.R. .
Source: Scientific Reports, 2016-07-07 00:00:00.0; 6, p. 29565.
EPub date: 2016-07-07 00:00:00.0.
PMID: 27383905
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Sequence-Specific Recognition of MicroRNAs and Other Short Nucleic Acids with Solid-State Nanopores.
Authors: Zahid O.K. , Wang F. , Ruzicka J.A. , Taylor E.W. , Hall A.R. .
Source: Nano Letters, 2016-03-09 00:00:00.0; 16(3), p. 2033-9.
EPub date: 2016-03-09 00:00:00.0.
PMID: 26824296
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