Grant Number:	5R01CA138836-03 Interpret this number
Primary Investigator:	Plon, Sharon
Organization:	Baylor College Of Medicine
Project Title:	Genomic Approaches to Defining Inherited Basis of Childhood Cancer
Fiscal Year:	2012

Identification of mutations in specific genes responsible for cancer susceptibility impacts decisions about treatment of the patient as well as allowing increased surveillance and prevention approaches for at-risk family members. In addition, knowledge of inherited predisposition to pediatric malignancies has provided important insights into the mechanisms of cancer and potential therapeutic targets in both children and adults with cancer in the general population. Current utilization of high-throughput sequencing methodologies for coding changes has been focused on somatic mutations in tumor tissue, and conversely; genome-wide association studies of cancer susceptibility have focused on common variations that individually have small impacts on cancer risk. We propose a project at the intersection of these two different approaches. We plan to use massively parallel sequence analyses of constitutional DNA from childhood cancer patients with medical and family history suggestive of a cancer susceptibility syndrome. In Aim 1 we will focus on cohorts of families with consistent and specific phenotypes, for example, probands with childhood sarcomas and second malignancies by age 40 where thorough analysis of well-characterized cancer-associated genes has been negative. The molecular analyses of germline DNA will include two comprehensive approaches (1) capture of all coding exons followed by sequencing at dense coverage to identify mutations pathogenic mutations (deletion, nonsense, frameshift or splice site) that impact coding sequence (whole exome) and (2) whole genome sequencing of paired-ends libraries at lower coverage to identify copy number changes and novel rearrangements. In Aim 2, we will carry out informative statistical and bioinformatics analysis of rare or novel missense alleles, using original methodology involving optimization of alignment depths for evolution-based studies of functionality. In Aim 3, analyses of copy number change and rearrangement by whole genome sequencing will include an additional cohort of subjects with childhood cancer and congenital anomalies or developmental delay to uncover novel contiguous gene or rearrangement syndromes. Results from these initial cohorts will then be validated by targeted sequencing of implicated genes in additional childhood cancer probands and families. To complete this translational, cross-disciplinary project we include collaborators from pediatric cancer genetics programs at Texas Children's Hospital, University of Texas MD Anderson Cancer Center and Health Science Center at San Antonio and the Children's Hospital of Philadelphia, the Human Genome Sequencing Center and the Department of Statistics at Rice University. The clinical centers enroll families from ethnically diverse populations with linkage to medical data, and establishment of immortalized cell lines to facilitate the generation of functionally and clinically relevant data. Our plan to thoroughly interrogate the constitutional genome will advance the goals of the National Cancer Institute's Strategic Plan on gaining a fuller understanding of the spectrum of genetic susceptibility to cancer in highly affected childhood cancer families. 1




Framework for microRNA variant annotation and prioritization using human population and disease datasets.
Authors: Oak N. , Ghosh R. , Huang K.L. , Wheeler D.A. , Ding L. , Plon S.E. .
Source: Human mutation, 2019 Jan; 40(1), p. 73-89.
EPub date: 2018-11-08.
PMID: 30302893
Related Citations

Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease.
Authors: van der Crabben S.N. , Hennus M.P. , McGregor G.A. , Ritter D.I. , Nagamani S.C. , Wells O.S. , Harakalova M. , Chinn I.K. , Alt A. , Vondrova L. , et al. .
Source: The Journal of clinical investigation, 2016-08-01; 126(8), p. 2881-92.
EPub date: 2016-07-18.
PMID: 27427983
Related Citations

Pharmacogenetic characterization of naturally occurring germline NT5C1A variants to chemotherapeutic nucleoside analogs.
Authors: Saliba J. , Zabriskie R. , Ghosh R. , Powell B.C. , Hicks S. , Kimmel M. , Meng Q. , Ritter D.I. , Wheeler D.A. , Gibbs R.A. , et al. .
Source: Pharmacogenetics and genomics, 2016 Jun; 26(6), p. 271-9.
PMID: 26906009
Related Citations

SV-STAT accurately detects structural variation via alignment to reference-based assemblies.
Authors: Davis C.F. , Ritter D.I. , Wheeler D.A. , Wang H. , Ding Y. , Dugan S.P. , Bainbridge M.N. , Muzny D.M. , Rao P.H. , Man T.K. , et al. .
Source: Source code for biology and medicine, 2016; 11, p. 8.
EPub date: 2016-06-18.
PMID: 27330550
Related Citations

Identifying gene disruptions in novel balanced de novo constitutional translocations in childhood cancer patients by whole-genome sequencing.
Authors: Ritter D.I. , Haines K. , Cheung H. , Davis C.F. , Lau C.C. , Berg J.S. , Brown C.W. , Thompson P.A. , Gibbs R. , Wheeler D.A. , et al. .
Source: Genetics in medicine : official journal of the American College of Medical Genetics, 2015 Oct; 17(10), p. 831-5.
EPub date: 2015-01-08.
PMID: 25569436
Related Citations

Targeted sequencing in chromosome 17q linkage region identifies familial glioma candidates in the Gliogene Consortium.
Authors: Jalali A. , Amirian E.S. , Bainbridge M.N. , Armstrong G.N. , Liu Y. , Tsavachidis S. , Jhangiani S.N. , Plon S.E. , Lau C.C. , Claus E.B. , et al. .
Source: Scientific reports, 2015-02-05; 5, p. 8278.
EPub date: 2015-02-05.
PMID: 25652157
Related Citations

Germline mutations in shelterin complex genes are associated with familial glioma.
Authors: Bainbridge M.N. , Armstrong G.N. , Gramatges M.M. , Bertuch A.A. , Jhangiani S.N. , Doddapaneni H. , Lewis L. , Tombrello J. , Tsavachidis S. , Liu Y. , et al. .
Source: Journal of the National Cancer Institute, 2015 Jan; 107(1), p. 384.
EPub date: 2014-12-07.
PMID: 25482530
Related Citations

A recurrent germline PAX5 mutation confers susceptibility to pre-B cell acute lymphoblastic leukemia.
Authors: Shah S. , Schrader K.A. , Waanders E. , Timms A.E. , Vijai J. , Miething C. , Wechsler J. , Yang J. , Hayes J. , Klein R.J. , et al. .
Source: Nature genetics, 2013 Oct; 45(10), p. 1226-1231.
EPub date: 2013-09-08.
PMID: 24013638
Related Citations

Identification of TP53 as an acute lymphocytic leukemia susceptibility gene through exome sequencing.
Authors: Powell B.C. , Jiang L. , Muzny D.M. , Treviño L.R. , Dreyer Z.E. , Strong L.C. , Wheeler D.A. , Gibbs R.A. , Plon S.E. .
Source: Pediatric blood & cancer, 2013 Jun; 60(6), p. E1-3.
EPub date: 2012-12-19.
PMID: 23255406
Related Citations

Unusually early presentation of small-bowel adenocarcinoma in a patient with Peutz-Jeghers syndrome.
Authors: Wangler M.F. , Chavan R. , Hicks M.J. , Nuchtern J.G. , Hegde M. , Plon S.E. , Thompson P.A. .
Source: Journal of pediatric hematology/oncology, 2013 May; 35(4), p. 323-8.
PMID: 23426006
Related Citations

Incidental copy-number variants identified by routine genome testing in a clinical population.
Authors: Boone P.M. , Soens Z.T. , Campbell I.M. , Stankiewicz P. , Cheung S.W. , Patel A. , Beaudet A.L. , Plon S.E. , Shaw C.A. , McGuire A.L. , et al. .
Source: Genetics in medicine : official journal of the American College of Medical Genetics, 2013 Jan; 15(1), p. 45-54.
EPub date: 2012-08-09.
PMID: 22878507
Related Citations

Constitutional tandem duplication of 9q34 that truncates EHMT1 in a child with ganglioglioma.
Authors: Cheung H.C. , Yatsenko S.A. , Kadapakkam M. , Legay H. , Su J. , Lupski J.R. , Plon S.E. .
Source: Pediatric blood & cancer, 2012 May; 58(5), p. 801-5.
EPub date: 2011-06-16.
PMID: 21681934
Related Citations

Exploring concordance and discordance for return of incidental findings from clinical sequencing.
Authors: Green R.C. , Berg J.S. , Berry G.T. , Biesecker L.G. , Dimmock D.P. , Evans J.P. , Grody W.W. , Hegde M.R. , Kalia S. , Korf B.R. , et al. .
Source: Genetics in medicine : official journal of the American College of Medical Genetics, 2012 Apr; 14(4), p. 405-10.
EPub date: 2012-03-15.
PMID: 22422049
Related Citations

Insights into the pathogenesis and treatment of cancer from inborn errors of metabolism.
Authors: Erez A. , Shchelochkov O.A. , Plon S.E. , Scaglia F. , Lee B. .
Source: American journal of human genetics, 2011-04-08; 88(4), p. 402-21.
PMID: 21473982
Related Citations

Unifying cancer genetics.
Authors: Plon S.E. .
Source: Genetics in medicine : official journal of the American College of Medical Genetics, 2011 Mar; 13(3), p. 203-4.
PMID: 21311342
Related Citations

Phenotypic manifestations of copy number variation in chromosome 16p13.11.
Authors: Nagamani S.C. , Erez A. , Bader P. , Lalani S.R. , Scott D.A. , Scaglia F. , Plon S.E. , Tsai C.H. , Reimschisel T. , Roeder E. , et al. .
Source: European journal of human genetics : EJHG, 2011 Mar; 19(3), p. 280-6.
EPub date: 2010-12-08.
PMID: 21150890
Related Citations

Identification of genetic susceptibility to childhood cancer through analysis of genes in parallel.
Authors: Plon S.E. , Wheeler D.A. , Strong L.C. , Tomlinson G.E. , Pirics M. , Meng Q. , Cheung H.C. , Begin P.R. , Muzny D.M. , Lewis L. , et al. .
Source: Cancer genetics, 2011 Jan; 204(1), p. 19-25.
PMID: 21356188
Related Citations