Grant Details
Grant Number: |
1R01CA151393-01 Interpret this number |
Primary Investigator: |
Sears, Cynthia |
Organization: |
Johns Hopkins University |
Project Title: |
Enterotoxigenic Bacteroides Fragilis: a Bacterial Promoter of Colon Oncogenesis |
Fiscal Year: |
2010 |
Abstract
DESCRIPTION (provided by applicant): We have identified a human colon anaerobic bacterium, enterotoxigenic Bacteroides fragilis (ETBF), as a candidate etiologic agent for colon cancer and our murine models of ETBF colonization delineate a potential procarcinogenic immune pathway that ETBF induce. Just as the understanding of peptic ulcer disease and ensuing stomach cancer was transformed by the discovery of H. pylori, we hypothesize that ETBF, by secreting the potent B. fragilis toxin (BFT), precipitate procarcinogenic mucosal immune responses, thereby promoting formation of colon cancer. Our model does not propose to alter existing mutational paradigms of colon cancer but rather proposes that ETBF colonization is an integral mechanism accounting for the accumulation of genetic mutations necessary for colon carcinogenesis. This proposal will study the relationship between colon colonization by ETBF in humans, specific colon immune responses and, ultimately, colon cancer. Defining a microbial etiology for colon cancer has key implications as colon cancer is a major public health problem being the second leading cause of cancer death in the United States in women and men. A significant correlation between any two of the studied variables - ETBF colonization, colon immune responses and colon cancer -- will substantively alter current paradigms for the pathogenesis, prevention and therapy of colon cancer.
PUBLIC HEALTH RELEVANCE: Colon cancer is the second leading cause of cancer death for women and men. Current approaches to prevention of colon cancer are cumbersome and underutilized due to their expense and inconvenience. This project proposes that colon cancer is triggered by a common stool bacterium called enterotoxigenic Bacteroides fragilis (ETBF) and will test whether detection of ETBF and/or the colon immune response to ETBF provide new, easier approaches to the prevention of the morbidity and mortality due to colon cancer.
Publications
Human Colon Mucosal Biofilms and Murine Host Communicate via Altered mRNA and microRNA Expression during Cancer.
Authors: Tomkovich S.
, Gharaibeh R.Z.
, Dejea C.M.
, Pope J.L.
, Jiang J.
, Winglee K.
, Gauthier J.
, Newsome R.C.
, Yang Y.
, Fodor A.A.
, et al.
.
Source: Msystems, 2020-01-14 00:00:00.0; 5(1), .
EPub date: 2020-01-14 00:00:00.0.
PMID: 31937674
Related Citations
Human colon mucosal biofilms from healthy or colon cancer hosts are carcinogenic.
Authors: Tomkovich S.
, Dejea C.M.
, Winglee K.
, Drewes J.L.
, Chung L.
, Housseau F.
, Pope J.L.
, Gauthier J.
, Sun X.
, Mühlbauer M.
, et al.
.
Source: The Journal Of Clinical Investigation, 2019-03-11 00:00:00.0; 130, p. 1699-1712.
EPub date: 2019-03-11 00:00:00.0.
PMID: 30855275
Related Citations
Patients with familial adenomatous polyposis harbor colonic biofilms containing tumorigenic bacteria.
Authors: Dejea C.M.
, Fathi P.
, Craig J.M.
, Boleij A.
, Taddese R.
, Geis A.L.
, Wu X.
, DeStefano Shields C.E.
, Hechenbleikner E.M.
, Huso D.L.
, et al.
.
Source: Science (new York, N.y.), 2018-02-02 00:00:00.0; 359(6375), p. 592-597.
EPub date: 2018-02-01 00:00:00.0.
PMID: 29420293
Related Citations
High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia.
Authors: Drewes J.L.
, White J.R.
, Dejea C.M.
, Fathi P.
, Iyadorai T.
, Vadivelu J.
, Roslani A.C.
, Wick E.C.
, Mongodin E.F.
, Loke M.F.
, et al.
.
Source: Npj Biofilms And Microbiomes, 2017; 3, p. 34.
EPub date: 2017-11-29 00:00:00.0.
PMID: 29214046
Related Citations
Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation.
Authors: Fu K.
, Sun X.
, Wier E.M.
, Hodgson A.
, Liu Y.
, Sears C.L.
, Wan F.
.
Source: Elife, 2016-07-25 00:00:00.0; 5, .
EPub date: 2016-07-25 00:00:00.0.
PMID: 27458801
Related Citations
Redundant Innate and Adaptive Sources of IL17 Production Drive Colon Tumorigenesis.
Authors: Housseau F.
, Wu S.
, Wick E.C.
, Fan H.
, Wu X.
, Llosa N.J.
, Smith K.N.
, Tam A.
, Ganguly S.
, Wanyiri J.W.
, et al.
.
Source: Cancer Research, 2016-04-15 00:00:00.0; 76(8), p. 2115-24.
EPub date: 2016-04-15 00:00:00.0.
PMID: 26880802
Related Citations
Do biofilms confer a pro-carcinogenic state?
Authors: Dejea C.M.
, Sears C.L.
.
Source: Gut Microbes, 2016; 7(1), p. 54-7.
PMID: 26939852
Related Citations
The intestinal microbiome and health.
Authors: Tuddenham S.
, Sears C.L.
.
Source: Current Opinion In Infectious Diseases, 2015 Oct; 28(5), p. 464-70.
PMID: 26237547
Related Citations
Fecal detection of enterotoxigenic Bacteroides fragilis.
Authors: Chen L.A.
, Van Meerbeke S.
, Albesiano E.
, Goodwin A.
, Wu S.
, Yu H.
, Carroll K.
, Sears C.
.
Source: European Journal Of Clinical Microbiology & Infectious Diseases : Official Publication Of The European Society Of Clinical Microbiology, 2015 Sep; 34(9), p. 1871-7.
PMID: 26173688
Related Citations
Metabolism links bacterial biofilms and colon carcinogenesis.
Authors: Johnson C.H.
, Dejea C.M.
, Edler D.
, Hoang L.T.
, Santidrian A.F.
, Felding B.H.
, Ivanisevic J.
, Cho K.
, Wick E.C.
, Hechenbleikner E.M.
, et al.
.
Source: Cell Metabolism, 2015-06-02 00:00:00.0; 21(6), p. 891-7.
EPub date: 2015-06-02 00:00:00.0.
PMID: 25959674
Related Citations
The Bacteroides fragilis toxin gene is prevalent in the colon mucosa of colorectal cancer patients.
Authors: Boleij A.
, Hechenbleikner E.M.
, Goodwin A.C.
, Badani R.
, Stein E.M.
, Lazarev M.G.
, Ellis B.
, Carroll K.C.
, Albesiano E.
, Wick E.C.
, et al.
.
Source: Clinical Infectious Diseases : An Official Publication Of The Infectious Diseases Society Of America, 2015-01-15 00:00:00.0; 60(2), p. 208-15.
EPub date: 2015-01-15 00:00:00.0.
PMID: 25305284
Related Citations
The vigorous immune microenvironment of microsatellite instable colon cancer is balanced by multiple counter-inhibitory checkpoints.
Authors: Llosa N.J.
, Cruise M.
, Tam A.
, Wicks E.C.
, Hechenbleikner E.M.
, Taube J.M.
, Blosser R.L.
, Fan H.
, Wang H.
, Luber B.S.
, et al.
.
Source: Cancer Discovery, 2015 Jan; 5(1), p. 43-51.
PMID: 25358689
Related Citations
Microbiota organization is a distinct feature of proximal colorectal cancers.
Authors: Dejea C.M.
, Wick E.C.
, Hechenbleikner E.M.
, White J.R.
, Mark Welch J.L.
, Rossetti B.J.
, Peterson S.N.
, Snesrud E.C.
, Borisy G.G.
, Lazarev M.
, et al.
.
Source: Proceedings Of The National Academy Of Sciences Of The United States Of America, 2014-12-23 00:00:00.0; 111(51), p. 18321-6.
EPub date: 2014-12-23 00:00:00.0.
PMID: 25489084
Related Citations
Bacteroides fragilis subverts mucosal biology: from symbiont to colon carcinogenesis.
Authors: Sears C.L.
, Geis A.L.
, Housseau F.
.
Source: The Journal Of Clinical Investigation, 2014 Oct; 124(10), p. 4166-72.
PMID: 25105360
Related Citations
A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes.
Authors: Dutilh B.E.
, Cassman N.
, McNair K.
, Sanchez S.E.
, Silva G.G.
, Boling L.
, Barr J.J.
, Speth D.R.
, Seguritan V.
, Aziz R.K.
, et al.
.
Source: Nature Communications, 2014-07-24 00:00:00.0; 5, p. 4498.
EPub date: 2014-07-24 00:00:00.0.
PMID: 25058116
Related Citations
Microbiota and immune responses in colon cancer: more to learn.
Authors: McAllister F.
, Housseau F.
, Sears C.L.
.
Source: Cancer Journal (sudbury, Mass.), 2014 May-Jun; 20(3), p. 232-6.
PMID: 24855013
Related Citations
Microbes, microbiota, and colon cancer.
Authors: Sears C.L.
, Garrett W.S.
.
Source: Cell Host & Microbe, 2014-03-12 00:00:00.0; 15(3), p. 317-28.
PMID: 24629338
Related Citations
Bacterial oncogenesis in the colon.
Authors: Dejea C.
, Wick E.
, Sears C.L.
.
Source: Future Microbiology, 2013 Apr; 8(4), p. 445-60.
PMID: 23534358
Related Citations
Shift from pStat6 to pStat3 predominance is associated with inflammatory bowel disease-associated dysplasia.
Authors: Wick E.C.
, LeBlanc R.E.
, Ortega G.
, Robinson C.
, Platz E.
, Pardoll D.M.
, Iacobuzio-Donahue C.
, Sears C.L.
.
Source: Inflammatory Bowel Diseases, 2012 Jul; 18(7), p. 1267-74.
PMID: 22021169
Related Citations
Perspective: alpha-bugs, their microbial partners, and the link to colon cancer.
Authors: Sears C.L.
, Pardoll D.M.
.
Source: The Journal Of Infectious Diseases, 2011-02-01 00:00:00.0; 203(3), p. 306-11.
EPub date: 2011-02-01 00:00:00.0.
PMID: 21208921
Related Citations