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Grant Details

Grant Number: 5R03CA097723-03 Interpret this number
Primary Investigator: Hurtubise, Robert
Organization: University Of Wyoming
Project Title: Solid-Matrix Luminescence of Polycyclic Aromatic Hydroc*
Fiscal Year: 2004
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Abstract

DESCRIPTION (provided by applicant)The objectives of the research are: (1) to develop a fundamental understanding of the photophysics for the solid-matrix luminescence (SML) of the highly toxic benzo[a]pyrene (B[a]P)-DNA adducts, dibenzo[a,l]pyrene (DB[a,l]P)-DNA adducts, and both polycyclic aromatic hydrocarbons (PAH) adducts bonded to the same sample of DNA so the basic principles developed can be broadly exploited analytically for a variety of multiple adducted PAH-DNA samples; (2) to elucidate the fundamental photophysical principles of the heavy-atom effect with solid-matrices to characterize B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and the two PAH adducts bonded to the same sample of DNA; (3) to lower the limits of detection for the SML of the B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and both adducts bonded to DNA at the levels found in most human samples; (4) to demonstrate that SML can be used to characterize and detect PAH-DNA adducts in purified blood samples from workers exposed to used motor oil and in purified placental DNA samples. The expected benefits from the research will be: (1) a fundamental foundation of photophysical knowledge for SML that could be applied broadly to divergent PAH-DNA adducts; (2) highly selective, sensitive, and relatively inexpensive SML methods for the direct characterization of the PAH-DNA adducts individually and in multiple forms; (3) several methods that would be very effective for cancer research related to B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and DNA samples bonded with different types of PAH which are important in assessing the environmental dangers from PAH.

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Publications

The characterization of (+/-)-anti-benzo[a]pyrene diolepoxide-DNA adducts and (+/-)-anti-dibenzo[a,l]pyrene diolepoxide--DNA adducts in the same DNA sample using solid-matrix phosphorescence.
Authors: Thompson A.L. , Hurtubise R.J. .
Source: Analytica chimica acta, 2007-02-12; 584(1), p. 28-36.
EPub date: 2006-11-10.
PMID: 17386581
Related Citations

Solid-matrix fluorescence quenching of benzo[e]pyrene and (+/-)-anti-dibenzo[a, l]pyrene diolepoxide-DNA adducts.
Authors: Thompson A.L. , Hurtubise R.J. .
Source: Applied spectroscopy, 2005 Jan; 59(1), p. 126-33.
PMID: 15720748
Related Citations




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