DESCRIPTION (provided by applicant):
The long-term goal of this research project is to study relationships between
exposure to fumonisins and microcystins and human liver and esophageal cancer
risks in high-risk populations. Fumonisins and microcystins are newly
identified environmental biotoxins that are produced by toxicogenic fungi and
cyanobacteria, respectively. Fumonisins are carcinogens and both fumonisins
and microcystins are strong tumor promoters in animal models. Human
populations in certain areas of world are exposed to higher levels of these
toxins in their daily life mainly through contaminated dietary components
and/or drinking water. Both of these toxins have been etiologically linked to
high incidence of human primary liver cancer and esophageal cancer in several
areas of South Africa and China. Although analytical methods for detecting
these toxins in environmental samples have been reported and potential
biomarkers, such as disruption of sphingolipids metabolism by fumonisins and
inhibition of protein phosphatases by microcystins, have been found in animal
models, validation of these biomarkers in humans, especially in high-risk
populations, has not been done or reported. To date, methods are still lack
for simultaneously detection of these toxins in environmental samples and
human body fluids, which are critical for assessment of human cancer risks,
because co-exposure to these biotoxins in high-risk populations has been
widely reported. In this exploratory research project, we will develop and
validate analytical array methods to simultaneously measure these biotoxins in
both environmental samples and human body fluids. We will use molecular
epidemiological tools to validate methods for biomarkers in body fluids of
cases of primary liver cancer and esophageal cancer and controls. The working
hypothesis underlying this research proposal is that long-term exposure to
fumonisins and microcystins may induce synergistic carcinogenic effects in
high-risk individuals and application of validated biomarkers can improve the
quantitative estimation of human cancer risk from exposure to these biotoxins.
The specific aims are: 1) to develop rapid and sensitive analytical array
method(s) for measuring fumonisin and microcystin biomarkers based on
previously developed immunoaffinity-HPLC method for aflatoxins and/or
HPLC-enzyme-linked immunosorbent assay for microcystins; 2) to validate
method(s) for measuring biomarkers of microcystins and fumonisins in food,
water, and body fluids such as blood and urine samples collected from two
one-week longitudinal biomonitoring studies in human subjects from high-risk
areas of primary liver cancer and esophageal cancer, and 3) to perform
molecular epidemiological studies in two high-risk populations of primary
liver cancer and esophageal cancer in Qidong and Huaian, P.R. China, for
exploring cancer risks from exposures to fumonisins and microcystins.
If you are accessing this page during weekend or evening hours, the database may currently be offline for maintenance and should operational within a few hours. Otherwise, we have been notified of this error and will be addressing it immediately.
Please contact us
if this error persists.
We apologize for the inconvenience.
- The DCCPS Team.