Grant Details
Grant Number: |
5R01CA064247-09 Interpret this number |
Primary Investigator: |
Ho, Gloria |
Organization: |
Albert Einstein College Of Medicine |
Project Title: |
Spontaneous Regression Biomarkers in Cervix Dysplasia |
Fiscal Year: |
2003 |
Abstract
DESCRIPTION: (Adapted from the Investigator's Abstract) The applicants propose
to continue an ongoing prospective study in which women with CIN I or II are
recruited and followed in order to identify biomarkers associated with
regression of CIN. The following factors will be assessed: (1) humoral immune
response to virus-like particles (VLPs) of HPV types 6, 16, 18, 31, 53, and 58,
(2) cell-mediated immunity (CMI) to HPV 16 E6 and E7 peptides, (3) class II HLA
DQB1 and DRB1 alleles, (4) plasma ascorbic acid level, and (5) red blood cell
(RBC) folate level. Continuation of this existing study is necessary to
increase sample size and thus provide sufficient statistical power to better
understand how these host factors may interact to influence the outcome of CIN.
Women will be recruited from the colposcopy clinics of 3 hospitals associated
with the Albert Einstein College of Medicine and followed at 3-month intervals
by Pap smear and colposcopy for 12 months. At this point, an endpoint biopsy
will be performed to determine whether CIN lesions are present ('persistence')
or absent ('regression'). At each visit, cervico-vaginal lavage specimens will
be collected for HPV DNA analyses by Southern blot and polymerase chain
reaction (PCR). Blood will be collected for HPV VLP serology, T cell
proliferative response (CMI) assay, plasma reduced ascorbic acid by high
pressure liquid chromatography, and RBC folate levels by immunoassay. DNA
typing of class II HLA alleles by PCR will be performed using blood collected
at baseline. Associations between these factors and regression of CIN will be
assessed by appropriate univariate and multivariate analyses.
Publications
Risk factors for persistent cervical intraepithelial neoplasia grades 1 and 2: managed by watchful waiting.
Authors: Ho G.Y.
, Einstein M.H.
, Romney S.L.
, Kadish A.S.
, Abadi M.
, Mikhail M.
, Basu J.
, Thysen B.
, Reimers L.
, Palan P.R.
, et al.
.
Source: Journal of lower genital tract disease, 2011 Oct; 15(4), p. 268-75.
PMID: 21811178
Related Citations
Variability of serum levels of tumor necrosis factor-alpha, interleukin 6, and soluble interleukin 6 receptor over 2 years in young women.
Authors: Ho G.Y.
, Xue X.N.
, Burk R.D.
, Kaplan R.C.
, Cornell E.
, Cushman M.
.
Source: Cytokine, 2005-04-07; 30(1), p. 1-6.
PMID: 15784406
Related Citations
Polymorphism of the insulin gene is associated with increased prostate cancer risk.
Authors: Ho G.Y.
, Melman A.
, Liu S.M.
, Li M.
, Yu H.
, Negassa A.
, Burk R.D.
, Hsing A.W.
, Ghavamian R.
, Chua S.C.
.
Source: British journal of cancer, 2003-01-27; 88(2), p. 263-9.
PMID: 12610512
Related Citations
Regression of cervical intraepithelial neoplasia and loss of human papillomavirus (HPV) infection is associated with cell-mediated immune responses to an HPV type 16 E7 peptide.
Authors: Kadish A.S.
, Timmins P.
, Wang Y.
, Ho G.Y.
, Burk R.D.
, Ketz J.
, He W.
, Romney S.L.
, Johnson A.
, Angeletti R.
, et al.
.
Source: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 2002 May; 11(5), p. 483-8.
PMID: 12010863
Related Citations
Transmission/disequilibrium tests of androgen receptor and glutathione S-transferase pi variants in prostate cancer families.
Authors: Ho G.Y.
, Knapp M.
, Freije D.
, Nelson W.G.
, Smith J.R.
, Carpten J.D.
, Bailey-Wilson J.E.
, Beaty T.H.
, Petersen G.
, Xu J.
, et al.
.
Source: International journal of cancer, 2002-04-20; 98(6), p. 938-42.
PMID: 11948476
Related Citations
PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems.
Authors: Qu W.
, Jiang G.
, Cruz Y.
, Chang C.J.
, Ho G.Y.
, Klein R.S.
, Burk R.D.
.
Source: Journal of clinical microbiology, 1997 Jun; 35(6), p. 1304-10.
PMID: 9163434
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