Grant Details
Grant Number: |
1R03CA097723-01 Interpret this number |
Primary Investigator: |
Hurtubise, Robert |
Organization: |
University Of Wyoming |
Project Title: |
Solid-Matrix Luminescence of Polycyclic Aromatic Hydroc* |
Fiscal Year: |
2002 |
Abstract
DESCRIPTION (provided by applicant)The objectives of the research are: (1) to develop a fundamental understanding of the photophysics for the solid-matrix luminescence (SML) of the highly toxic benzo[a]pyrene (B[a]P)-DNA adducts, dibenzo[a,l]pyrene (DB[a,l]P)-DNA adducts, and both polycyclic aromatic hydrocarbons (PAH) adducts bonded to the same sample of DNA so the basic principles developed can be broadly exploited analytically for a variety of multiple adducted PAH-DNA samples; (2) to elucidate the fundamental photophysical principles of the heavy-atom effect with solid-matrices to characterize B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and the two PAH adducts bonded to the same sample of DNA; (3) to lower the limits of detection for the SML of the B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and both adducts bonded to DNA at the levels found in most human samples; (4) to demonstrate that SML can be used to characterize and detect PAH-DNA adducts in purified blood samples from workers exposed to used motor oil and in purified placental DNA samples.
The expected benefits from the research will be: (1) a fundamental foundation of photophysical knowledge for SML that could be applied broadly to divergent PAH-DNA adducts; (2) highly selective, sensitive, and relatively inexpensive SML methods for the direct characterization of the PAH-DNA adducts individually and in multiple forms; (3) several methods that would be very effective for cancer research related to B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and DNA samples bonded with different types of PAH which are important in assessing the environmental dangers from PAH.
Publications
The Characterization Of (+/-)-anti-benzo[a]pyrene Diolepoxide-dna Adducts And (+/-)-anti-dibenzo[a,l]pyrene Diolepoxide--dna Adducts In The Same Dna Sample Using Solid-matrix Phosphorescence
Authors: Thompson A.L.
, Hurtubise R.J.
.
Source: Analytica Chimica Acta, 2007-02-12 00:00:00.0; 584(1), p. 28-36.
PMID: 17386581
Related Citations
Solid-matrix Fluorescence Quenching Of Benzo[e]pyrene And (+/-)-anti-dibenzo[a, L]pyrene Diolepoxide-dna Adducts
Authors: Thompson A.L.
, Hurtubise R.J.
.
Source: Applied Spectroscopy, 2005 Jan; 59(1), p. 126-33.
PMID: 15720748
Related Citations