Grant Details
Grant Number: |
7R01CA068565-05 Interpret this number |
Primary Investigator: |
Schwartz, Gary |
Organization: |
Wake Forest University Health Sciences |
Project Title: |
Prostate Cancer & Vitamin D-Receptors, Responses & Race |
Fiscal Year: |
1998 |
Abstract
The Principal lnvestigator and colleagues have pioneered the hypothesis
that deficiencies of 1,25-Dihydroxyvitamin D (1,25 D) increase the risk
for clinical prostate cancer. This hypothesis led us to investigate the
effects of vitamin D on established prostate cancer cell lines. We found
that cells from an invasive prostate cancer cell line did not respond to
vitamin D despite the presence of vitamin D receptors; whereas, a non-
invasive prostate cancer cell line was sensitive to 1 ,25 D. This
suggests that a deficient response to 1,25 D may be a characteristic of
invasive prostate cancer cells. We will test the hypothesis that invasive
prostate cancer cells exhibit diminished biochemical response to vitamin
D compared to non-cancer prostate cells. Our Specific Aims are to: 1.
ascertain vitamin D receptor levels and vitamin D responsiveness in
invasive prostate cancer cells and in non-cancer prostate cells; 2.
determine how these receptor levels and responses differ among men from
3 racial/ethnic groups at different risk for prostate cancer.
In order to realize these aims we will: 1. Obtain surgical specimens of
fresh prostatic tissue from Black, White, and Hispanic prostate cancer
patients undergoing radical prostatectomy at the University of
Miami/Jackson Memorial Medical Center; 2. Prepare primary cultures of
invasive prostate cancer and non-cancer prostate cells from each patient
using an in vitro cell invasion assay; 3. Assess vitamin D receptor
levels and vitamin D receptor function by reporter gene assay. Our
primary goal is to determine whether vitamin D sensitivity differentiates
invasive prostate cancer from non-cancerous prostate cells. This research
may ultimately permit the identification of those prostate cancers that
are likely to be invasive. The identification of invasive prostate cancer
is critical in determining which men should receive aggressive treatment
for their disease.
Publications
Vitamin D and the epidemiology of prostate cancer.
Authors: Schwartz G.G.
.
Source: Seminars In Dialysis, 2005 Jul-Aug; 18(4), p. 276-89.
PMID: 16076349
Related Citations
The prostate 25-hydroxyvitamin D-1 alpha-hydroxylase is not influenced by parathyroid hormone and calcium: implications for prostate cancer chemoprevention by vitamin D.
Authors: Young M.V.
, Schwartz G.G.
, Wang L.
, Jamieson D.P.
, Whitlatch L.W.
, Flanagan J.N.
, Lokeshwar B.L.
, Holick M.F.
, Chen T.C.
.
Source: Carcinogenesis, 2004 Jun; 25(6), p. 967-71.
PMID: 14729578
Related Citations
Evaluation of vitamin D analogs as therapeutic agents for prostate cancer.
Authors: Chen T.C.
, Holick M.F.
, Lokeshwar B.L.
, Burnstein K.L.
, Schwartz G.G.
.
Source: Recent Results In Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer, 2003; 164, p. 273-88.
PMID: 12899529
Related Citations
The G gamma / T-15 transgenic mouse model of androgen-independent prostate cancer: target cells of carcinogenesis and the effect of the vitamin D analogue EB 1089.
Authors: Perez-Stable C.M.
, Schwartz G.G.
, Farinas A.
, Finegold M.
, Binderup L.
, Howard G.A.
, Roos B.A.
.
Source: Cancer Epidemiology, Biomarkers & Prevention : A Publication Of The American Association For Cancer Research, Cosponsored By The American Society Of Preventive Oncology, 2002 Jun; 11(6), p. 555-63.
PMID: 12050097
Related Citations
25-Hydroxyvitamin D-1alpha-hydroxylase activity is diminished in human prostate cancer cells and is enhanced by gene transfer.
Authors: Whitlatch L.W.
, Young M.V.
, Schwartz G.G.
, Flanagan J.N.
, Burnstein K.L.
, Lokeshwar B.L.
, Rich E.S.
, Holick M.F.
, Chen T.C.
.
Source: The Journal Of Steroid Biochemistry And Molecular Biology, 2002 Jun; 81(2), p. 135-40.
PMID: 12137802
Related Citations
The in vitro evaluation of 25-hydroxyvitamin D3 and 19-nor-1alpha,25-dihydroxyvitamin D2 as therapeutic agents for prostate cancer.
Authors: Chen T.C.
, Schwartz G.G.
, Burnstein K.L.
, Lokeshwar B.L.
, Holick M.F.
.
Source: Clinical Cancer Research : An Official Journal Of The American Association For Cancer Research, 2000 Mar; 6(3), p. 901-8.
PMID: 10741714
Related Citations
Inhibition of prostate cancer metastasis in vivo: a comparison of 1,23-dihydroxyvitamin D (calcitriol) and EB1089.
Authors: Lokeshwar B.L.
, Schwartz G.G.
, Selzer M.G.
, Burnstein K.L.
, Zhuang S.H.
, Block N.L.
, Binderup L.
.
Source: Cancer Epidemiology, Biomarkers & Prevention : A Publication Of The American Association For Cancer Research, Cosponsored By The American Society Of Preventive Oncology, 1999 Mar; 8(3), p. 241-8.
PMID: 10090302
Related Citations
1 alpha,25-Dihydroxyvitamin D (calcitriol) inhibits the invasiveness of human prostate cancer cells.
Authors: Schwartz G.G.
, Wang M.H.
, Zang M.
, Singh R.K.
, Siegal G.P.
.
Source: Cancer Epidemiology, Biomarkers & Prevention : A Publication Of The American Association For Cancer Research, Cosponsored By The American Society Of Preventive Oncology, 1997 Sep; 6(9), p. 727-32.
PMID: 9298581
Related Citations