||5R03CA074284-02 Interpret this number
||University Of Utah
||Detecting Antineoplastic Drugs
DESCRIPTION: (Applicant's Description) This study proposes research on the
exposure of health care workers to the drug cyclophosphamide, which is a
human carcinogen and which is used extensively in cancer chemotherapy.
Nurses and pharmacists who handle or administer antineoplastic drugs have
been shown to have detectable amounts in urine at the end of the work shift,
despite the use of biosafety hoods, gloves, and recommended work practices.
Because end-of-shift urine measurements assess low level exposures only
during the previous 1-3 days, and because spills and unplanned exposures
occur sporadically, a method that assesses exposure over a longer period
would be more useful in assessing effectiveness of work practices and other
preventive measures. Hair analysis has been used extensively for detection
of trace quantities of illicit drugs and some therapeutic drugs and current
technology allows detection at the nanogram of drug/mg. of hair level. A
method will be developed for assay of cyclophosphamide and a major
metabolite, carboxyphosphamide, in hair. The method will be validated and a
dose-response relationship established in rats. A pilot study of nurses and
pharmacists who work with cyclophosphamide, and a control group, will then
be done to assess exposure. Detection of the metabolite in human hair will
distinguish internal absorption from external contamination from the work
environment. If this pilot study is successful a larger study could be done
of health care workers at various cancer treatment centers. In addition to
assisting epidemiologic research, the proposed method would be a useful
means of assessing and controlling exposure to antineoplastic drugs.
Strain-specific differences in formation of apoptotic DNA ladders in MCF-7 breast cancer cells.
, Yee D.
Cancer letters, 1999-09-20; 144(1), p. 31-7.