Grant Details
Grant Number: |
5R35CA253187-03 Interpret this number |
Primary Investigator: |
Couch, Fergus |
Organization: |
Mayo Clinic Rochester |
Project Title: |
Resolving the Cancer Relevance of Predisposition Gene Mutations |
Fiscal Year: |
2022 |
Abstract
Breast cancer has a strong heritable component with approximately 15% of patients exhibiting a family history
of the disease. My group recently established that inherited variants in 12 genes (ATM, BARD1, BRCA1, BRCA2,
CDH1, CHEK2, NF1, PALB2, PTEN, RAD51C, RAD51D, and TP53) predispose to breast cancer (1, 2), that
variants in all 12 genes increase risks of breast cancer in minority populations (3), and that variants in certain
genes predispose only to estrogen receptor (ER) positive (ATM and CHEK2) or ER negative and triple negative
breast cancer (TNBC) (BARD1, RAD51C and RAD51D) (4-6). Despite these major advances, clinical application
of the information is still lacking. In addition, up to 50% of the familial risk of breast cancer remains unexplained.
Under this award we plan to address clinically relevant issues, including improved application of genetic testing
results for risk management of patients and improved selection of breast cancer therapy. In addition, we aim to
identify new breast cancer predisposition genes that account for the missing heritability. The proposed studies
are unified under a theme of advancing understanding of predisposition genetics. The studies are as follows:
A. Age-specific and population-specific cancer risk assessment for predisposition gene variants. Results from
hereditary multigene panel testing has limited clinical utility because only lifetime risk estimates of cancer by age
80 are available. Here we will estimate 5 and 10-year risks of breast cancer, so that patients can make decisions
about medical management. In addition, we have evidence that specific genes have much higher penetrance in
African Americans. We will determine the penetrance of predisposition gene variants using a large African
American cohort study in order to modify risk management guidelines for this population.
B. Functional characterization of predisposition gene variants. Variants of uncertain significance (VUS)
identified by genetic testing remain a major problem for individuals receiving clinical genetic testing. We aim to
combine high-throughput functional analysis of VUS in ATM, BRCA2 and PALB2 genes with genetic data from
families in integrated models to determine the clinical relevance of many VUS alterations.
C. Therapeutic response for breast cancer predisposition genes. The responsiveness of breast tumors
associated with predisposition gene variants to standard or targeted therapy is only known for BRCA1 and
BRCA2 mutation carriers. Here we aim to identify all patients with pathogenic variants in the commonly mutated
BRCA1, BRCA2, PALB2, ATM and CHEK2 genes from a series of neo-adjuvant, adjuvant and metastatic breast
cancer clinical trials and to assess response to therapy and outcome.
D. Identification of novel breast cancer predisposition alleles. The common and rare risk alleles for breast
cancer account for only 50% of the familial risk in the population. In an effort to identify the missing heritability
we will collaborate with Regeneron Inc. through our SIMPLEXO consortium to identify common and rare alleles
associated with breast cancer risk in 45,000 breast cancer patients.
Publications
Genetic Risk of Second Primary Cancer in Breast Cancer Survivors: The Multiethnic Cohort Study.
Authors: Chen F.
, Park S.L.
, Wilkens L.R.
, Wan P.
, Hart S.N.
, Hu C.
, Yadav S.
, Couch F.J.
, Conti D.V.
, de Smith A.J.
, et al.
.
Source: Cancer research, 2022-09-16; 82(18), p. 3201-3208.
PMID: 35834270
Related Citations
Classification of BRCA2 Variants of Uncertain Significance (VUS) Using an ACMG/AMP Model Incorporating a Homology-Directed Repair (HDR) Functional Assay.
Authors: Hu C.
, Susswein L.R.
, Roberts M.E.
, Yang H.
, Marshall M.L.
, Hiraki S.
, Berkofsky-Fessler W.
, Gupta S.
, Shen W.
, Dunn C.A.
, et al.
.
Source: Clinical cancer research : an official journal of the American Association for Cancer Research, 2022-09-01; 28(17), p. 3742-3751.
PMID: 35736817
Related Citations
An integrative model for the comprehensive classification of BRCA1 and BRCA2 variants of uncertain clinical significance.
Authors: Iversen E.S.
, Lipton G.
, Hart S.N.
, Lee K.Y.
, Hu C.
, Polley E.C.
, Pesaran T.
, Yussuf A.
, LaDuca H.
, Chao E.
, et al.
.
Source: NPJ genomic medicine, 2022-06-03; 7(1), p. 35.
EPub date: 2022-06-03.
PMID: 35665744
Related Citations
Mapping molecular subtype specific alterations in breast cancer brain metastases identifies clinically relevant vulnerabilities.
Authors: Cosgrove N.
, Varešlija D.
, Keelan S.
, Elangovan A.
, Atkinson J.M.
, Cocchiglia S.
, Bane F.T.
, Singh V.
, Furney S.
, Hu C.
, et al.
.
Source: Nature communications, 2022-01-26; 13(1), p. 514.
EPub date: 2022-01-26.
PMID: 35082299
Related Citations
Rare germline copy number variants (CNVs) and breast cancer risk.
Authors: Dennis J.
, Tyrer J.P.
, Walker L.C.
, Michailidou K.
, Dorling L.
, Bolla M.K.
, Wang Q.
, Ahearn T.U.
, Andrulis I.L.
, Anton-Culver H.
, et al.
.
Source: Communications biology, 2022-01-18; 5(1), p. 65.
EPub date: 2022-01-18.
PMID: 35042965
Related Citations
Germline Pathogenic Variants in Cancer Predisposition Genes Among Women With Invasive Lobular Carcinoma of the Breast.
Authors: Yadav S.
, Hu C.
, Nathanson K.L.
, Weitzel J.N.
, Goldgar D.E.
, Kraft P.
, Gnanaolivu R.D.
, Na J.
, Huang H.
, Boddicker N.J.
, et al.
.
Source: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 2021-12-10; 39(35), p. 3918-3926.
EPub date: 2021-10-21.
PMID: 34672684
Related Citations
Risk of Late-Onset Breast Cancer in Genetically Predisposed Women.
Authors: Boddicker N.J.
, Hu C.
, Weitzel J.N.
, Kraft P.
, Nathanson K.L.
, Goldgar D.E.
, Na J.
, Huang H.
, Gnanaolivu R.D.
, Larson N.
, et al.
.
Source: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 2021-11-01; 39(31), p. 3430-3440.
EPub date: 2021-07-22.
PMID: 34292776
Related Citations
Germline variants and breast cancer survival in patients with distant metastases at primary breast cancer diagnosis.
Authors: Escala-Garcia M.
, Canisius S.
, Keeman R.
, Beesley J.
, Anton-Culver H.
, Arndt V.
, Augustinsson A.
, Becher H.
, Beckmann M.W.
, Behrens S.
, et al.
.
Source: Scientific reports, 2021-10-05; 11(1), p. 19787.
EPub date: 2021-10-05.
PMID: 34611289
Related Citations
Racial and Ethnic Differences in Multigene Hereditary Cancer Panel Test Results for Women With Breast Cancer.
Authors: Yadav S.
, LaDuca H.
, Polley E.C.
, Hu C.
, Niguidula N.
, Shimelis H.
, Lilyquist J.
, Na J.
, Lee K.Y.
, Gutierrez S.
, et al.
.
Source: Journal of the National Cancer Institute, 2021-10-01; 113(10), p. 1429-1433.
PMID: 33146377
Related Citations
Risk of Breast Cancer Among Carriers of Pathogenic Variants in Breast Cancer Predisposition Genes Varies by Polygenic Risk Score.
Authors: Gao C.
, Polley E.C.
, Hart S.N.
, Huang H.
, Hu C.
, Gnanaolivu R.
, Lilyquist J.
, Boddicker N.J.
, Na J.
, Ambrosone C.B.
, et al.
.
Source: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 2021-08-10; 39(23), p. 2564-2573.
EPub date: 2021-06-08.
PMID: 34101481
Related Citations
Comparison of the Prevalence of Pathogenic Variants in Cancer Susceptibility Genes in Black Women and Non-Hispanic White Women With Breast Cancer in the United States.
Authors: Domchek S.M.
, Yao S.
, Chen F.
, Hu C.
, Hart S.N.
, Goldgar D.E.
, Nathanson K.L.
, Ambrosone C.B.
, Haiman C.A.
, Couch F.J.
, et al.
.
Source: JAMA oncology, 2021-07-01; 7(7), p. 1045-1050.
PMID: 34042955
Related Citations
Mutations in BRCA1/2 and Other Panel Genes in Patients With Metastatic Breast Cancer -Association With Patient and Disease Characteristics and Effect on Prognosis.
Authors: Fasching P.A.
, Yadav S.
, Hu C.
, Wunderle M.
, Häberle L.
, Hart S.N.
, Rübner M.
, Polley E.C.
, Lee K.Y.
, Gnanaolivu R.D.
, et al.
.
Source: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 2021-05-20; 39(15), p. 1619-1630.
EPub date: 2021-03-29.
PMID: 33780288
Related Citations
Strong functional data for pathogenicity or neutrality classify BRCA2 DNA-binding-domain variants of uncertain significance.
Authors: Richardson M.E.
, Hu C.
, Lee K.Y.
, LaDuca H.
, Fulk K.
, Durda K.M.
, Deckman A.M.
, Goldgar D.E.
, Monteiro A.N.A.
, Gnanaolivu R.
, et al.
.
Source: American journal of human genetics, 2021-03-04; 108(3), p. 458-468.
EPub date: 2021-02-19.
PMID: 33609447
Related Citations
A Population-Based Study of Genes Previously Implicated in Breast Cancer.
Authors: Hu C.
, Hart S.N.
, Gnanaolivu R.
, Huang H.
, Lee K.Y.
, Na J.
, Gao C.
, Lilyquist J.
, Yadav S.
, Boddicker N.J.
, et al.
.
Source: The New England journal of medicine, 2021-02-04; 384(5), p. 440-451.
EPub date: 2021-01-20.
PMID: 33471974
Related Citations